W10 original doesn't start after wintousb use

[martin.gale]

I cloned my w10 on a usb drive withouth  problem usi ng the automatic wintousb shutdown after cloning .  Then, I intent to start  on the usb drive..it works. I shutdowned again and rebooted on my internal drive.....no boot sector. I intended to repair the boot with w10 DVD.. it doesn't work. I don't know what can I do now. ..